Aim:
To run a Gel Electrophoresis Kit.
Research:
Theory:
DNA is a ….. made up of 4 smaller nucleotide units, which are abbreviated…..
These units connect in specific pairs, …. connects with …., and ….. connects with …..
The DNA we used for this experiment is from ….. In its linear form. Lambda is a virus. Viruses work by….
Restriction enzymes are specialised at cutting DNA and recognise…
Gel Electrophoresis uses …….,
And so because the backbones of the DNA strands are negatively charged, when you put a positive electrode at the far end of the gel to the wells, they will want to move through the gel.
The Gel runs for 10 minutes, and then we look at the stripes that the DNA lengths make in the Gel.
Method:
We practised by using the ….., putting dots of coloured water onto paper sheets. Then we did the experiment.
**Copy (in your own words) instructions 1-6 on Page 7 of the booklet.**
We had 5 samples,
U label means Uncut, and was put in well number ….
H label means ……., and was put in well number ….
E label means ……., and was put in well number ….
D label means ……., and was put in well number ….
L label means for ……, and was put in well number ….
Results:
Predicted Results:
Copy the table from Page 6 in the booklet (photocopied as your handout).
Actual Results:
Photos of your complete Gel.
Discussion:
- Some discussion questions